The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional buffers. The gel is placed next to the membrane and the application of an electrical current. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). The Power Blotter System achieves highly efficient transfer in seven minutes when used with Power Blotter Select Transfer Stacks or ten minutes with Power Blotter 1-Step Transfer Buffer. Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The easy-touch programming feature allows custom transfer settings to be quickly created, saved, and run. The included Power Blotter Station has an easy-to-use color LCD touchscreen interface with pre-programmed transfer methods for different numbers and sizes of gels and ranges of molecular weight protein. The Power Blotter Cassette, included in the Power Blotter System, enables the simultaneous transfer of up to two mini-size or one midi-size gel. Learn more about the semi-dry transfer device: Power Blotter System Versatile-compatible with Power Blotter Select Transfer Stacks and Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods.Flexible-simultaneous transfer of 1 to 2 mini-size or 1 midi-size gel using the Power Blotter Cassette.Easy-touch programming-convenient pre-programmed transfer methods or create, save, and run customized transfer methods.Integrated power supply-seamless operation between control unit and cassette no need for external power supply.Fast-5 to 12 minute transfer when used with Power Blotter Select Transfer Stacks or Power Blotter 1-Step Transfer Buffer.Efficient-high transfer efficiency with a broad range of protein sizes compared to conventional semi-dry or wet (tank) transfer methods.The Power Blotter features an integrated power supply optimized to enable consistent and high-efficiency protein transfer.įeatures of the Power Blotter System include: The Invitrogen Power Blotter System is designed specifically for rapid semi-dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 12 minutes.
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